Has anyone used Zymo DNA RNA shield for extractions from saliva?

I want to collect saliva samples in the Zymo DNA RNA shield in order to extract human DNA and RNA from the saliva. Has anyone tried this, either DNA, RNA or preferably both, and was the quality...

08 September 2019 7,806 1 View

How do I verify age and language capabilities when making use of data collection via online questionnaires?

Our inclusion criteria are that participants must be 18 years or older and that they must be able to read and understand English/Afrikaans in order to take part in the study. However, the informed...

02 March 2018 6,995 3 View

Suggestions for reliable online questionnaires to evaluate neuropsychiatric disorder symptoms?

Can anyone recommend validated online (web-based) questionnaires to evaluate neuropsychiatric disorders/symptoms?

09 October 2017 425 4 View

Are there labs in Lyon, France, working on neuroscience & epigenetics?

I will be visiting Lyon in October and is interested to visit some labs there that preferably work in the behavioural neuroscience/neuro genetics/microbiome/transciptomics/epigenetics fields. Just...

08 September 2014 6,684 5 View

Which kits are best to use for isolating microbial DNA from stool samples?

06 July 2014 5,655 15 View

What is the best protocol for extraction of high quality RNA from Oragene samples?

We are having trouble extracting RNA from Oragene samples; we have very low 260:230 ratios and certain samples have very low concentrations. We have tried the RNAeasy kit, the trizol and a...

01 February 2014 6,115 2 View

Dilutions of precursor miRNA and 3'UTR target for co-transfection in luciferase experiment?

I want to make sure if my calculations are correct. I have 370ng/ul stock of my 3'UTR reporter plasmid, I have to use 10ng during the transfection, does that mean that I use 0.03ul of the stock...

07 August 2013 8,419 6 View

Should I differentiate my neuroscreen-1 cells before conducting functional miRNA analyses?

I have neuroscreen-1 (NS-1) cells (theses cell are sub-clones from PC12 cells) which I want to co-transfect with a specific miRNA as well as that miRNAs predicted mRNA target, to see if the miRNA...

06 July 2013 7,728 11 View

Products for functional miRNA experiments

Which products or brands would you recommend for performing miRNA luciferase assays to determine if an mRNA sequence is a direct target of the particular miRNA? Specifically relating to the miRNA...

05 June 2013 6,348 1 View

Program to predict CpG islands in gene bodies.

I would like to know what prediction programs are out there that predict CpG islands within gene bodies (not promoters).

01 February 2013 8,611 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View