We are having trouble extracting RNA from Oragene samples; we have very low 260:230 ratios and certain samples have very low concentrations. We have tried the RNAeasy kit, the trizol and a combinational of the RNAeasy and trizol kits. Does anyone have any suggestion on how to improve the quality, as we are planning on using these samples for RNAseq and real-time PCR analyses?
Thanks Rohan, I will forward your response to our research assistant who is performing our extractions. With low concentrations I mean like between 6ng/ul - 20ng/ul for certain samples, according to the Nanodrop. Apparently the RNA extracted from Oragene samples do not work well on the bioanalyzer, or have you tried it before? Is there another way of determining the quality?
Yes, we have quantified it on the qubit as well, but the service provider only sent us the concentrations, so I am not sure if it also provides info on quality and purity.
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