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Questions related from Stefanie Malan
I want to collect saliva samples in the Zymo DNA RNA shield in order to extract human DNA and RNA from the saliva. Has anyone tried this, either DNA, RNA or preferably both, and was the quality...
09 September 2019 7,871 1 View
Our inclusion criteria are that participants must be 18 years or older and that they must be able to read and understand English/Afrikaans in order to take part in the study. However, the informed...
03 March 2018 7,059 3 View
Can anyone recommend validated online (web-based) questionnaires to evaluate neuropsychiatric disorders/symptoms?
10 October 2017 493 4 View
I will be visiting Lyon in October and is interested to visit some labs there that preferably work in the behavioural neuroscience/neuro genetics/microbiome/transciptomics/epigenetics fields. Just...
09 September 2014 6,772 5 View
We are planning on doing microbiome analyses with next generation sequencing and would like to know what kits we should use for collection and extraction of microbial DNA?
07 July 2014 5,718 15 View
We are having trouble extracting RNA from Oragene samples; we have very low 260:230 ratios and certain samples have very low concentrations. We have tried the RNAeasy kit, the trizol and a...
02 February 2014 6,175 2 View
I want to make sure if my calculations are correct. I have 370ng/ul stock of my 3'UTR reporter plasmid, I have to use 10ng during the transfection, does that mean that I use 0.03ul of the stock...
08 August 2013 8,480 6 View
I have neuroscreen-1 (NS-1) cells (theses cell are sub-clones from PC12 cells) which I want to co-transfect with a specific miRNA as well as that miRNAs predicted mRNA target, to see if the miRNA...
07 July 2013 7,788 11 View
Which products or brands would you recommend for performing miRNA luciferase assays to determine if an mRNA sequence is a direct target of the particular miRNA? Specifically relating to the miRNA...
06 June 2013 6,405 1 View
I would like to know what prediction programs are out there that predict CpG islands within gene bodies (not promoters).
02 February 2013 8,674 2 View
Has anyone freeze-dried their miRNA samples (in a separate small RNA fraction, not in total RNA) in order to increase the concentration? Does it affect the RNA integrity?
11 November 2012 442 14 View
I've seen numerous articles showing good correlation between their qPCR and transcriptome seq data, however, I have not had such great correlation. Is this more common than most articles report,...
10 October 2012 6,801 15 View
Has anyone used the Apollo324 kit for miRNA seq experiments? Apparently it works well with small amounts of starting material. I would also like to know if anyone has used a small RNA fraction (
08 August 2012 8,220 1 View
I have very low concentrations of miRNAs within my small RNA fraction that I want to sequence on the Illumina HiScanSQ. The Illumina protocol states that 1ug of total RNA should be used as input,...
07 July 2012 1,887 1 View
I would like to know what the general consensus is regarding cut-off values for gene expression fold changes (is it mainly >2 up and down-regulated?). Also, is this cut-off applied together with...
07 July 2012 4,519 11 View
