Does anyone have the normal human mammary luminal epithelial cells (primary or cell line)?

There are many luminal breast cancer cell lines (MCF7, T47D etc). I was wondering whether there is anything like normal human mammary luminal cell line.

08 September 2014 7,531 28 View

Which normalization method is better for microRNA microarray data analysis?

Recently we performed miRNA microarray and we found that many novel miRNA candidates are present in our data. Although many of them have high expression value, their functions are still undefined...

07 August 2014 8,448 6 View

Is investigating cellular quiescence a 'waste of time' in cancer therapy?

Say, if you thought its a tumor suppressor gene, but found it induces quiescence. Is it better not to invest much time on it?

04 May 2014 4,158 9 View

What are the good markers for G2/M phase of cell cycle?

I was getting high G2/M population in PI staining. I was looking for a specific marker (FC/IF/WB) of G2/M transition. Cyclin B1 and CDK1 usually acts in this stage. Can anyone please suggest a marker?

03 April 2014 4,873 14 View

Is Epithelial-mesenchymal transition (EMT) a dedifferentiated state of the cell?

EMT generate cells with stem like properties. I was wondering whether it reverses the the differentiated cells into undifferentiated state? My cells undergo dedifferentiation and acquires cancer...

02 March 2014 3,277 4 View

Microarray data analysis - how to analyze two conditions if one lacks expression value?

I have two conditions, untreated and drug treated. Some genes only express in response to drug treatment. So, I didn't get their expression in untreated condition and hence I could not calculate...

01 February 2014 2,005 9 View

Is there any in vitro system to demonstrate metastatic colonization?

What should I do before mouse experiment?

10 November 2013 1,598 9 View

Is there an easy way to retrieve gene expression profile along with survival information from Gene Expression Omnibus (GEO) database?

Any program to further process SOFT formatted or Series matrix file?

09 October 2013 2,062 4 View

Has anyone used doxycycline-induced microRNA for 3`-UTR luciferase assay rather than mimic?

I`m getting 50-100 fold induction of my desired miRNA by inducible system. I was curious about the extent of reduction of luciferase activity.

09 October 2013 3,195 3 View

Has anyone designed the shRNA targeting a particular microRNA, just like the 'mirZIP' from systems bioscience?

I think mirZIP from systems bioscience is not inducible. I was thinking to make it inducible by cloning into knockout inducible RNAi system (Clontech). Just wondering how many bp mismatches are...

06 July 2013 674 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View