Ruhul Amin

13 Questions 81 Answers 0 Followers

Questions related from Ruhul Amin

Does anyone have the normal human mammary luminal epithelial cells (primary or cell line)?

There are many luminal breast cancer cell lines (MCF7, T47D etc). I was wondering whether there is anything like normal human mammary luminal cell line.

09 September 2014 7,622 28 View

Which normalization method is better for microRNA microarray data analysis?

Recently we performed miRNA microarray and we found that many novel miRNA candidates are present in our data. Although many of them have high expression value, their functions are still undefined...

08 August 2014 8,541 6 View

Is investigating cellular quiescence a 'waste of time' in cancer therapy?

Say, if you thought its a tumor suppressor gene, but found it induces quiescence. Is it better not to invest much time on it?

05 May 2014 4,248 9 View

What are the good markers for G2/M phase of cell cycle?

I was getting high G2/M population in PI staining. I was looking for a specific marker (FC/IF/WB) of G2/M transition. Cyclin B1 and CDK1 usually acts in this stage. Can anyone please suggest a marker?

04 April 2014 4,963 14 View

Is Epithelial-mesenchymal transition (EMT) a dedifferentiated state of the cell?

EMT generate cells with stem like properties. I was wondering whether it reverses the the differentiated cells into undifferentiated state? My cells undergo dedifferentiation and acquires cancer...

03 March 2014 3,378 4 View

Microarray data analysis - how to analyze two conditions if one lacks expression value?

I have two conditions, untreated and drug treated. Some genes only express in response to drug treatment. So, I didn't get their expression in untreated condition and hence I could not calculate...

02 February 2014 2,092 9 View

Is there any in vitro system to demonstrate metastatic colonization?

What should I do before mouse experiment?

11 November 2013 1,685 9 View

Has anyone used doxycycline-induced microRNA for 3`-UTR luciferase assay rather than mimic?

I`m getting 50-100 fold induction of my desired miRNA by inducible system. I was curious about the extent of reduction of luciferase activity.

10 October 2013 3,274 3 View

Is there an easy way to retrieve gene expression profile along with survival information from Gene Expression Omnibus (GEO) database?

Any program to further process SOFT formatted or Series matrix file?

10 October 2013 2,161 4 View

Has anyone designed the shRNA targeting a particular microRNA, just like the 'mirZIP' from systems bioscience?

I think mirZIP from systems bioscience is not inducible. I was thinking to make it inducible by cloning into knockout inducible RNAi system (Clontech). Just wondering how many bp mismatches are...

07 July 2013 768 8 View

Does direction matter, like clockwise or counterclockwise (3'UTR cloning)?

I have cloned a 5 Kb 3'UTR of my GOI into a single XbaI site of a pRL-CMV vector. I want to see binding of microRNA with that but I got my insert in an opposite direction (luc-3' end of UTR--- 5'...

03 March 2013 8,945 8 View

Has anyone used RPMI medium for mammosphere formation assay?

Usually DMEM/F12 is recommended for Mammosphere formation assay. But I've grown some of my cell line in RPMI medium. I was wondering if I transfer them into DMEM/F12, how do they react? Is that OK...

03 March 2013 608 3 View

Is it possible for a single microRNA to act differently depending on cell types?

I mean within the same cancer but different stages ( primary, metastatic), can a single microRNA act as oncogenic or tumor suppressor?

01 January 2013 5,904 9 View