10 February 2018 2 8K Report

I believe RPMI and other culture media have high levels of glucose and that the concentration of oxygen used in incubators(21%) is supraphysiological compared to in tissues. Correct me if I’m wrong but were these conditions originally devised for cell lines? If so does anyone have any experience using different conditions which are more physiological and what are your experiences?

Thanks!

ben.

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