Ben Mulhearn

2 Questions 4 Answers 0 Followers

Questions related from Ben Mulhearn

Has anyone used more physiological culture medium and conditions for primary cells such as PBMCs?

I believe RPMI and other culture media have high levels of glucose and that the concentration of oxygen used in incubators(21%) is supraphysiological compared to in tissues. Correct me if I’m...

10 February 2018 8,095 2 View

Do monocytes in thawed PBMCs adhere if rested overnight?

I'm looking to isolate monocytes from thawed PBMCs, by negative selection using MACS. I usually rest overnight, but I'm worried that the monocytes will adhere to the culture plate. Does anyone...

28 September 2016 9,064 2 View