Why is the GFP expressed from my viral vector so weak?

I made a plant viral vector expressing GFP. I got this GFP from a previously made construct (a viral vector of the same type) which I PCR amplified the GFP from, and used restriction enzyme...

19 October 2020 2,863 3 View

Nicotiana benthamiana growth chamber conditions?

UPDATE: SEE MY LAST COMMENT FOR SOLUTION! I'm trying to figure out the best conditions to grow my N. benthamiana plants in a growth chamber. I will be Agro infiltrating them later, around 4...

11 November 2019 6,018 10 View

How to use biotinylated primary antibody; streptavidin APC secondary antibody in compensation beads to get positive population?

Hello, I am currently setting up my compensation matrix in flow cytometry and to do this I am using compensation beads (versacomp antibody capture beads) to get negative and positive population...

13 February 2019 2,224 4 View

How to measure complement receptor 1 per erythrocyte using flow cytometry?

Hello, I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has...

05 January 2019 5,345 3 View

Plant histology stains?

Hello, I'm looking for plant histology methods for use on traditional microscopes (we have a UV lamp) instead of confocal laser (too expensive for our lab to use) that would allow me to...

18 November 2018 7,647 1 View

Has anyone succesfully expressed Gq DREADDS in rats?

My lab is interested in using DREADDS (Gq) targeting excitatory neurons in rats, specifically Long Evans. Unfortunately, we have not been able to successfully express Gq in cell culture (mice) or...

22 June 2016 8,233 1 View

Can anyone tell me how to construct a taxonomic tree of all genera of proteobacteria?

I don't need to go to the species level, just the genus level, and I would like to make a hierarchal taxonomic tree to show from phylum (proteobacteria) down to each genus. My plan is to then...

24 July 2015 5,174 9 View

What are ict skills of preschool teachers?

I'm planning to do research on ict skills of preschool teachers. I am wondering what are the ict skills of preschool teachers.

08 February 2015 1,549 14 View

Does anyone have experience with the PCR conditions for maize invertase, NPTII, and Cry1A primers?

I have had no results for any of these primers and I used the calculated annealing temperatures for each. Does anyone have experience with these primers or have any suggestions?

18 July 2013 7,407 2 View

Can anyone share any fellowships, grants, or funding sources you know of for plant biology graduate students?

I'm trying to compile a list of funding opportunities for plant biology graduate students (for me, because I'm looking for funding, but also as a resource to share with others in the future). Does...

01 January 1970 7,116 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View