Why is the GFP expressed from my viral vector so weak?

I made a plant viral vector expressing GFP. I got this GFP from a previously made construct (a viral vector of the same type) which I PCR amplified the GFP from, and used restriction enzyme...

19 October 2020 2,863 3 View

Is Intechopen a reputable Publisher?

I have received numerous invites from IntechOpen to contribute to their books. Can anyone assist is this publisher reputable?

08 June 2020 4,217 3 View

How to perfom Unconflated Multilevel analysis?

I want to use Unconflated Multilevel Modeling (UMM) to test a 1-1-1 model in which the effects of the mediating and predictive variables can also be on level 2. Because of a small sample size (164...

24 December 2019 8,603 3 View

Nicotiana benthamiana growth chamber conditions?

UPDATE: SEE MY LAST COMMENT FOR SOLUTION! I'm trying to figure out the best conditions to grow my N. benthamiana plants in a growth chamber. I will be Agro infiltrating them later, around 4...

11 November 2019 6,018 10 View

How to use biotinylated primary antibody; streptavidin APC secondary antibody in compensation beads to get positive population?

Hello, I am currently setting up my compensation matrix in flow cytometry and to do this I am using compensation beads (versacomp antibody capture beads) to get negative and positive population...

13 February 2019 2,224 4 View

How to measure complement receptor 1 per erythrocyte using flow cytometry?

Hello, I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has...

05 January 2019 5,345 3 View

Plant histology stains?

Hello, I'm looking for plant histology methods for use on traditional microscopes (we have a UV lamp) instead of confocal laser (too expensive for our lab to use) that would allow me to...

18 November 2018 7,647 1 View

How to interpret the following mediation outcome (regression based)?

In my research I hypothised that team learning mediates between transformational leadership and innovative work behavior. To analyse the relations I use HLM 7. I found a significant positive...

25 January 2018 9,913 4 View

Has anyone succesfully expressed Gq DREADDS in rats?

My lab is interested in using DREADDS (Gq) targeting excitatory neurons in rats, specifically Long Evans. Unfortunately, we have not been able to successfully express Gq in cell culture (mice) or...

22 June 2016 8,233 1 View

How long can formalin-methanol fixed cells be in good condition for sorting and further RNA analysis?

18 February 2016 2,358 2 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

As the first flagelant confraternity is mentioned one in Perugia in 1260. But, some data testify such confraternity in 1214/Zadar and 1215/Dubrovnik?

Data from Zadar's archive mention the fraternity of flagellants (dei flagellanti) in Zadar in 1214founded in the church of St. Silvester (fratalia seu scola sancti Silvestri Iadere Frustancium)....

14 February 2021 3,136 2 View

What is corrct way to define ports when simulating FSS and surface waves in CST?

I need to simulate a mushroom FSS and I'm struggling with the ports. What's the correct way to define them? Should I construct a proper SMA connectors, or is it enough to put a waveguide ports...

19 January 2021 6,590 4 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Is religious education mandatory in public schools in your country? Can anyone provide some data or sources from the internacional context?

Religious education. Religion in public schools.

03 January 2021 7,097 25 View

No title

Kindly mention the insects and support it with a verse from the book of your religion. For atheists also which is your take?

31 December 2020 385 5 View

Impact of veil (hijab) and Nimaz (Five times prayers in Islam)??

It was observed globally that by putting mask on face reduced the transmission of coronavirus which is already stated in Islam. Moreover, by praying 5 times a days is essential in Islam in which...

06 December 2020 637 7 View

Hello, how can I invite an article?

Entrepreneurship in agriculture: a literature review by Roland Condor International Journal of Entrepreneurship and Small Business (IJESB), Vol. 40, No. 4, 2020

23 November 2020 5,898 13 View

The attitudes of religious groups towards the relations within a family?

I am looking for the most recent scientific findings concerning attitudes of religious groups towards the relations within a family (equality, subordination, pshysical violence toward wife or...

19 November 2020 6,416 4 View