Could cloudy, spreading growth seen in yeast plated on agar be contamination?

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Why is the GFP expressed from my viral vector so weak?

I made a plant viral vector expressing GFP. I got this GFP from a previously made construct (a viral vector of the same type) which I PCR amplified the GFP from, and used restriction enzyme...

19 October 2020 2,863 3 View

Nicotiana benthamiana growth chamber conditions?

UPDATE: SEE MY LAST COMMENT FOR SOLUTION! I'm trying to figure out the best conditions to grow my N. benthamiana plants in a growth chamber. I will be Agro infiltrating them later, around 4...

11 November 2019 6,018 10 View

How to use biotinylated primary antibody; streptavidin APC secondary antibody in compensation beads to get positive population?

Hello, I am currently setting up my compensation matrix in flow cytometry and to do this I am using compensation beads (versacomp antibody capture beads) to get negative and positive population...

13 February 2019 2,224 4 View

How to measure complement receptor 1 per erythrocyte using flow cytometry?

Hello, I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has...

05 January 2019 5,345 3 View

Plant histology stains?

Hello, I'm looking for plant histology methods for use on traditional microscopes (we have a UV lamp) instead of confocal laser (too expensive for our lab to use) that would allow me to...

18 November 2018 7,647 1 View

Has anyone succesfully expressed Gq DREADDS in rats?

My lab is interested in using DREADDS (Gq) targeting excitatory neurons in rats, specifically Long Evans. Unfortunately, we have not been able to successfully express Gq in cell culture (mice) or...

22 June 2016 8,233 1 View

Is that OK if I keep the buffer solely buffered by HEPES in the 5% CO2 incubator?

Buffer: 145mM NaCl, 5mM KCl, 1mM MgSO4, 10mM D-Glucose and 10mM HEPES, 2mM CaCl2, 2.5mM Probenecid. I just found from online someone said buffer solely buffered by HEPES should not be kept in CO2...

10 December 2015 8,421 3 View

Can anyone tell me how to construct a taxonomic tree of all genera of proteobacteria?

I don't need to go to the species level, just the genus level, and I would like to make a hierarchal taxonomic tree to show from phylum (proteobacteria) down to each genus. My plan is to then...

24 July 2015 5,174 9 View

What are ict skills of preschool teachers?

I'm planning to do research on ict skills of preschool teachers. I am wondering what are the ict skills of preschool teachers.

08 February 2015 1,549 14 View

How do we define the soluble proteins and insoluble proteins in cells? Is there any standard? Can anyone suggest me the corresponding lysis buffer?

I am working on separating the soluble and insoluble proteins in cells. However, after the literature searching, I found there are many different lysis buffers, which means the corresponding...

15 January 2015 4,641 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View