We used grape bagasse as a growth substrate for production of lignocellulolytic enzymes by white-rot fungi. Majority of them grew well in both submerged and solid-state fermentation in the presence of additional nitrogen source. However, enzyme activity usually was much lower as compared with substrates reach with cellulose.

Hi Iroakazi

We had several plant trash like fruit bunches, straws and other materials to transform the cellulosis in Bioethanol. You have more literature online explaining systems for enzyme productions based on solid bed fermentations of regular bagasse or cellulosic waste of industrial alcohol productions, usually a specialized reactor is required and a mechanical pretreatment like milling, or grinding to get finer sizes of the solid.

This can be difficult to carry out mechanically, we use Polytron dispergers, they have a big selection of switchable units for all kind of cuttings and homogenisations and he big unit has a maximum energy of 1,7 kw, you can even cut branches if you use the right unit