I am trying to establish a coculture of adult mice endothelial brain cells and newborn mice astrocytes for an in vitro model of blood brain barrier . The idea is to seed the astrocytes on the abluminal side of a matrigel covered transwell, while seeding endothelial cells on the luminal face of the insert. When I seed them separately, both cell types grow very well, however, when I establish the coculture, endothelial cells die.
Does anyone have experience in this type of cocultures?
Thank you very much for your help
We have previously grown rat astrocytes using our coculture system CRC (conditionally reprogrammed cells), In this case we used "fibroblasts conditioned media , so you could perhaps try an "endotelial conditioned media" instead of the physical barrier (transwell)? For our system, the results of transwell and conditioned media are just the same.
Thank you very much for your reply Nancy. Unfortunately, I must use a transwell because I need to carry out transmigration assays :-(
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