Has anyone successfully used newborn calf serum for culture of HEK293T cells?

25 October 2018 1 6K Report

Has anyone successfully used newborn calf serum in place of fetal bovine serum for culture of HEK293T cells? The cells will be used to produce a recombinant protein in serum-free media later on, but for the expansion and maintenance of the cells would be nice to use the inexpensive NBCS instead of FBS. I've heard some people reporting success but if anyone can confirm this serum is OK for HEK's it would be much appreciated. Thanks.

Zachery R Belak

Thanks Jiang Kunpeng

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