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Has anyone successfully used newborn calf serum in place of fetal bovine serum for culture of HEK293T cells? The cells will be used to produce a recombinant protein in serum-free media later on,...
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I have experience with 1H-15N HSQC of 15N enriched proteins. I have read some papers reporting natural abundance 1H-15N HSQC so it appears possible. I have a 19 kDa protein which I have isolated...
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