The Bleo treated mice have an increase in lung elastance (fibrosis) and do not develop emphysema.  Emphysema would decrease lung elastance (or increase compliance as E=1/C).  We could not get pirfenidone or nintedanib to decrease fibrosis in the bleo mouse model, but the ALK-5 inhibitor SB525334 (you can buy it from Sigma-Aldrich) works (see linked paper below).  Good luck with your research!

Article Bleomycin Induces Molecular Changes Directly Relevant to Idi...

We tried to set up the bleomycin model with limited success years ago. It is rather tricky and the bleo source is an important variable; the outcome might depend on it. I believe the best ("cleanest" fibrosis) was when using Blenoxane (Bristol-Meyers Squib). Check the paper below and multiple papers by Elizabeth Travis and Mike Blackburn - both their labs worked with bleo models quite a bit.

Bleomycin-Induced Chronic Lung Damage Does Not

Resemble Human Idiopathic Pulmonary Fibrosis

GISELLA BORZONE, RODRIGO MORENO, RODRIGO URREA, MANUEL MENESES, MANUEL OYARZÚN,

and CARMEN LISBOA

Thank you, is it possible to purchase from Bristol-Meyers Squib outside the US? I live in Israel. Thank you

My previous lab used to use this model in a routine basis. I'll see if I can find out where we biught ours for you and get you back.

You can buy the drug for research purposes from Abcam [Bleomycin sulfate (ab142977)], Sigma-Aldrich [We've used this one successfully in mice], or Cayman Chemical [Item № 13877].  CAUTION:  You should use C57Bl/6 mice, not BALB/c, for these experiments due to differential resistance to the drug.

In my previous lab, we had tried to developed Bleomycin induced fibrosis model in Beas2b cell line as well in mice using Bleomycin sulfate from sigma. We were able to developed this in cell line, but we struggle in mice model. So we tried silica induced fibrosis model, which is an alternate of bleomycin.