Has anyone measured GUS histochemical activity in imbibed arabidopsis thaliana seeds?

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José A Abelenda

Plant Molecular Biology Reporter June 2002, Volume 20, Issue 2, pp 107-114

It's easy but beware of false positives (e.g the recently appeared of incongruencies in some auxin related papers).

Rocío Soledad Tognacca

Thank you José! I´m sorry to ask, but what do you mean by false positives?

This protocol explains it perfectly:

http://microscopy.tamu.edu/lab-protocols/GUS_Localization_in_plants.pdf

One can be more confident in the results with some not so complicated controls.

Good luck!