In the mtb-infected mouse model, should I do the perfusion if I want to harvest the lung and monitor the organ CFU?

Normally, if we need to isolate the lymphocytes from the lungs, perfusion through the heart is necessary, by which the blood lymphocyte contamination will be excluded and less red blood cells...

10 November 2016 1,669 1 View

Is there anything in blood co-relative to the bacterial burden in MTB infected mice?

Hi guys, I am working with the mtb-infected mouse model. and i am thinking about whether we can monitor the bacterial burden with mouse blood. the current way we do for the bacterial burden...

09 October 2016 6,472 4 View

Have anyone tried the antibiotic combination of 7H11 selectable agar on plating mice organ homogenate?

Hi guys, I am here again with mycobacteria question. for plating organ homogenate from SPF mouse, we have to add antibiotic combination to the 7H11 agar. the combination I used for BalB/c mice...

09 October 2016 9,792 7 View

What is the sequence of wild type GP33 in LCMV?

08 September 2016 4,933 4 View

In terms of background, what should I start from as a beginner in immunological field?

I have worked with Mtb for 8 years, yet I am going to work in a lab focusing on immunology, apparently i will have a learning gap, what should I start from about CD8+ T cell response in mouse model?

07 August 2016 6,455 3 View

Got a math issue when i build the mutation library?

i am building a mutation library in Mycobacterium, and after i collected the colonies of the library, the total CFU will be more than 10^9 CFU. there are 4000 genes in the genome, so i can say...

02 March 2016 7,915 3 View

Is there any animals we already know having the resistance to mycobacterium tuberculosis?

08 September 2014 2,309 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View