Wenxi Xu

8 Questions 12 Answers 0 Followers

Questions related from Wenxi Xu

In the mtb-infected mouse model, should I do the perfusion if I want to harvest the lung and monitor the organ CFU?

Normally, if we need to isolate the lymphocytes from the lungs, perfusion through the heart is necessary, by which the blood lymphocyte contamination will be excluded and less red blood cells...

11 November 2016 1,732 1 View

Have anyone tried the antibiotic combination of 7H11 selectable agar on plating mice organ homogenate?

Hi guys, I am here again with mycobacteria question. for plating organ homogenate from SPF mouse, we have to add antibiotic combination to the 7H11 agar. the combination I used for BalB/c mice...

10 October 2016 9,860 7 View

Is there anything in blood co-relative to the bacterial burden in MTB infected mice?

Hi guys, I am working with the mtb-infected mouse model. and i am thinking about whether we can monitor the bacterial burden with mouse blood. the current way we do for the bacterial burden...

10 October 2016 6,560 4 View

What is the sequence of wild type GP33 in LCMV?

I have a question about the epitope GP33 of LCMV. In some paper, it is indicated that the wild type GP33-41 is KAVYNFATM, yet in others, the wild type GP33-41 turned to be KAVYNFATC. Which one is...

09 September 2016 4,995 4 View

In terms of background, what should I start from as a beginner in immunological field?

I have worked with Mtb for 8 years, yet I am going to work in a lab focusing on immunology, apparently i will have a learning gap, what should I start from about CD8+ T cell response in mouse model?

08 August 2016 6,525 3 View

Got a math issue when i build the mutation library?

i am building a mutation library in Mycobacterium, and after i collected the colonies of the library, the total CFU will be more than 10^9 CFU. there are 4000 genes in the genome, so i can say...

03 March 2016 8,010 3 View

Is there any animals we already know having the resistance to mycobacterium tuberculosis?

i am just curious

09 September 2014 2,369 0 View

Has anyone experienced mouse moulting on the head 11 weeks after being injected BCG ?

5 mice were injected with BCG, 11 week later, 4 moulted on the head.

09 September 2012 7,442 1 View