In the mtb-infected mouse model, should I do the perfusion if I want to harvest the lung and monitor the organ CFU?

Normally, if we need to isolate the lymphocytes from the lungs, perfusion through the heart is necessary, by which the blood lymphocyte contamination will be excluded and less red blood cells...

10 November 2016 1,669 1 View

Is there anything in blood co-relative to the bacterial burden in MTB infected mice?

Hi guys, I am working with the mtb-infected mouse model. and i am thinking about whether we can monitor the bacterial burden with mouse blood. the current way we do for the bacterial burden...

09 October 2016 6,472 4 View

Have anyone tried the antibiotic combination of 7H11 selectable agar on plating mice organ homogenate?

Hi guys, I am here again with mycobacteria question. for plating organ homogenate from SPF mouse, we have to add antibiotic combination to the 7H11 agar. the combination I used for BalB/c mice...

09 October 2016 9,792 7 View

What is the sequence of wild type GP33 in LCMV?

08 September 2016 4,933 4 View

In terms of background, what should I start from as a beginner in immunological field?

I have worked with Mtb for 8 years, yet I am going to work in a lab focusing on immunology, apparently i will have a learning gap, what should I start from about CD8+ T cell response in mouse model?

07 August 2016 6,455 3 View

Got a math issue when i build the mutation library?

i am building a mutation library in Mycobacterium, and after i collected the colonies of the library, the total CFU will be more than 10^9 CFU. there are 4000 genes in the genome, so i can say...

02 March 2016 7,915 3 View

Has anyone experienced mouse moulting on the head 11 weeks after being injected BCG ?

5 mice were injected with BCG, 11 week later, 4 moulted on the head.

08 September 2012 7,376 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View