i am just curious
Normally, if we need to isolate the lymphocytes from the lungs, perfusion through the heart is necessary, by which the blood lymphocyte contamination will be excluded and less red blood cells...
10 November 2016 1,767 1 View
Hi guys, I am working with the mtb-infected mouse model. and i am thinking about whether we can monitor the bacterial burden with mouse blood. the current way we do for the bacterial burden...
09 October 2016 6,615 4 View
Hi guys, I am here again with mycobacteria question. for plating organ homogenate from SPF mouse, we have to add antibiotic combination to the 7H11 agar. the combination I used for BalB/c mice...
09 October 2016 9,902 7 View
I have a question about the epitope GP33 of LCMV. In some paper, it is indicated that the wild type GP33-41 is KAVYNFATM, yet in others, the wild type GP33-41 turned to be KAVYNFATC. Which one is...
08 September 2016 5,027 4 View
I have worked with Mtb for 8 years, yet I am going to work in a lab focusing on immunology, apparently i will have a learning gap, what should I start from about CD8+ T cell response in mouse model?
07 August 2016 6,567 3 View
i am building a mutation library in Mycobacterium, and after i collected the colonies of the library, the total CFU will be more than 10^9 CFU. there are 4000 genes in the genome, so i can say...
02 March 2016 8,054 3 View
5 mice were injected with BCG, 11 week later, 4 moulted on the head.
08 September 2012 7,469 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...
10 July 2024 7,457 1 View
If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...
03 July 2024 2,371 1 View
We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...
01 July 2024 2,686 2 View
thanks
25 June 2024 4,667 0 View
Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...
23 June 2024 4,763 0 View
23 June 2024 6,583 2 View
HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...
18 June 2024 5,741 0 View
I read a paper where they converted WGS to core genome to check for AMR through resfinder, any insights would be appreciated, new to bioinformatics.
10 June 2024 8,347 1 View
