Change unit from mV to mAU on HPLC?

More Ambreen Latif's questions See All

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

How do I categories into groups on SPSS?

I am using the 'Revised Cyber Bullying Inventory'. This instrument has 2 sub-scales (bully scale and victim scale). From the responses, they will be divided into 4 groups (pure cyberbullies, pure...

13 February 2021 5,787 4 View

Which is the best wavelength to study aerosol optical depth(AOD) related to climate reseach,which criteria must be adopted in choosing the wavelengh?

We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...

12 February 2021 6,324 3 View

How to correctly reference instruments in systematic review following APA?

Hello, I am running into an issue and do not know how to cite / reference correctly here. APA states that instruments and measurements need to be referenced with the author's name. At the same...

11 February 2021 9,523 3 View

Rousseau Psychological Contract instrument?

Hi, I would appreciated if someone who's studying the Psychological contrat could help me about this questionnaire : My question is the following : Is someone can help me identify how wich...

09 February 2021 9,162 3 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

How to resolve a corrupted .odb file?

Hello. I run my Abaqus simulation in the supercomputer and once it's done, I copied the result (odb file) into my local computer using winSCP. But I faced a problem to read that file (see...

08 February 2021 6,198 3 View

Michele Raucci

The unit of measurement depends on the instrument connected to the computer running the software. It is the software that determines which scale to use. The manual states this (see page 100).

https://resources.perkinelmer.com/corporate/cmsresources/images/46-74577man_totalchromworkstationvolume1.pdf

This is a conversion from an "electrical signal" as volts to "spectral signal" as absorbance units AU.

However, if you need it, you may derive the conversion factor empirically by plotting the voltage signal vs the absorbance value and acquiring the slope value for one of your Standard solutions. Set the Y-axis to readout as millivolts, run the standard sample; then set the y-axix to readout as AU, then run the STD. Plot 'millivolts' vs 'AU'. The value of the slope would be your empirically derived conversion factor.

Qamar Ul Islam

Ambreen Latif To change the unit from "mV" to "mAU" on your PerkinElmer HPLC instrument's TotalChrom Navigator software for plotting mAU versus concentration, you can follow these steps:

1. Open the TotalChrom Navigator software on your computer.

2. Load or open the chromatogram or data file you want to work with.

3. In the software's main interface, locate the settings or options menu. It may be labeled as "Instrument," "Data Analysis," or something similar.

4. Within the settings or options menu, look for a section related to signal or detector settings. This is where you can configure how the data is displayed.

5. In the signal or detector settings, you should find an option to change the unit of measurement. It may be labeled as "Units" or "Signal Units."

6. Select "mAU" (milli-absorbance units) as the desired unit for your detector or signal.

7. Save or apply the changes.

Once you have changed the unit to "mAU," your plot should display absorbance in milli-absorbance units versus concentration. Make sure to check your instrument's user manual or software documentation for specific instructions tailored to your PerkinElmer HPLC system and software version, as menu options may vary slightly depending on the software version and instrument model.