Has anyone ever isolated RNA from cells lysed with RLT Plus buffer (QIAGEN) using the Zymo Research Rapid RNA isolation kit?

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Laurence Stuart Dawkins-Hall

Dear Margarita

in my experience these lysis buffers tend to be similar with Guanidium salts as there main active principle

This I would go ahead and purify the lysate with the Zymo kit and you will probably be ok

Margarita Dominguez-Villar

Dear Laurence,

we tried the isolation today and it worked as well as with the lysis buffer Zymo Research provides. Thanks for your suggestion.

Good news Margarita