What is the most suitable dye to stain fungal structures inside the plant tissue?

More Edgar Villar-Luna's questions See All

Any homemade protocol to extract genomic DNA from leaf litter?

We are trying to extract DNA from ant nests to amplify the 16S gene, but apparently, the extraction allows the passage of PCR inhibitors. Thank you for your comments

15 July 2024 9,314 2 View

How to avoid SS contamination in RF magnetron sputtering?

Hello I am encountering Fe and other stainless steel (SS) element contamination in my LiCoO2 RF sputtering depositions. I suspect the source is the "etching" of the magnetron stainless steel frame...

11 June 2024 2,196 3 View

No correlation between the independent variables and the dependent variable?

I want to conduct a multivariable linear regression analysis with 15 independent variables, however when I was checking the assumptions not a single IV correlates with the dependent variable. Can...

14 May 2024 5,151 7 View

Can anyone help me with names of software that I can use to model the water quality of a spring?

I'm doing a project that involves modelling the water quality of a spring but the programs I've come across only deal with lakes and rivers. So any suggestions?

10 May 2024 2,897 3 View

Can anyone help me with designing for a flat roof's drainage to bs en 12056?

Attached is the structure in question

09 April 2024 1,020 1 View

Best IQ measure to assess suspected intellectual disability?

I am a psychologist in the United States (in Florida, specifically). I conduct a variety of evaluations. Occasionally, I receive referrals for IQ and academic achievement testing with school-aged...

26 February 2024 2,579 3 View

What is the correct way to calculte the wave lenght of visible light in metals?

This should be a simple question. Given the real part of the refractive index n′ for Silver, approximately 0.05, and applying the relationship for the wavelength inside a material, λ=λ0/n',...

08 February 2024 3,247 1 View

Saving the publication list?

How can I save the list of publications to an external location? Best regards Edgar Selzer

02 November 2023 1,957 5 View

How much PCR product do I have to use for a Cas9 digestion?

I needed to do a Cas9 digestion of the amplicon, but I did not achieve it. Some protocols refer to the use of 1-2 uL of PCR product directly with optional purification, but I do not understand why...

16 September 2023 3,526 0 View

How to interpret the strength of association of the biserial correlation (rb)?

Hello, I estimated the biserial correlation between an artificial dichotomous variable and a continuous variable. But I want to know how to interpret the result in terms of strength of...

13 September 2023 2,528 0 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How Can I Apply Hashin Damage Properties to Solid Elements in Abaqus Without Using VUMAT?

Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...

28 July 2024 8,169 0 View

Will the catalytic effect of retained austenite happens in steels including RA?

The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...

27 July 2024 8,508 0 View

Arvind Singh

Please follow the links given below.

https://www.researchgate.net/post/which_stain_useful_in_differentiating_fungal_hyphae_form_lignocellulosic_biomass

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC90956/

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2011.02462.x/pdf

https://www.researchgate.net/publication/237166119_Visualization_of_mycorrhizal_fungal_structures_in_resin_embedded_tissues_with_anthene_dyes_using_laser_scanning_confocal_microscopy

Good luck

Article Visualization of mycorrhizal fungal structures in resin embe...

Andrew Taylor

We use WGA (https://www.thermofisher.com/order/catalog/product/W11261) which works well for roots.

Please check this pdf attachment also.

Elena Yu. Voronina

Dear Edgar,

Any blue stains work well. For mycorrhizal structures chlorazol black E is often applied. Useful information you can find at Mark Brundrett`s site http://mycorrhizas.info/method.html. Besides see a paper attached on ink and vinegar stain.

Best regards,

Elena