According to JAX, it is not recommended to use male 3xTg mice as tested subjects because of the report that male transgenic mice may not exhibit the phenotypic traits originally described. But for eletrophysiological test, male mice are usually preferred due to little estrogen effect. So is it a good method to test their LTP? Thanks a lot!
No myself but I know that A. Gruart from Pablo de Olavide University has performed LTP experiments in 3xTg mice. Her e-mail is [email protected]. I think the paper is published with Sanfeliu as corresponding author from Barcelona.
LTP:
[1] S. Oddo, A. Caccamo, J.D. Shepherd, M.P. Murphy, T.E. Golde, R. Kayed, R. Metherate, M.P. Mattson, Y. Akbari, F.M. LaFerla, Triple-transgenic model of Alzheimer’s disease with plaques and tangles: intracellular Abeta and synaptic dysfunction, Neuron 39 (2003) 409–421.
Intrinsic /active / synaptic properties :
[1] D. Arsenault, A. Dal-Pan, C. Tremblay, D.A. Bennett, M.J. Guitton, Y. De Koninck, S. Tonegawa, F. Calon, PAK inactivation impairs social recognition in 3xTg-AD Mice without increasing brain deposition of tau and Abeta., J Neurosci 33 (2013) 10729–10740.
[2] D. Arsenault, C. Julien, C. Tremblay, F. Calon, DHA Improves Cognition and Prevents Dysfunction of Entorhinal Cortex Neurons in 3xTg-AD Mice., PLoS One 6 (2011) e17397.
Several groups have. I think Frank LaFerla's group was the initial group in about 2002 or 2003. Do a quick Pubmed or GoogleScholar search and you should find that paper.
We also ran several electrophysiological experiments in the 3XTg mice, including both extracellular and intracellular recordings at the University of Kentucky in the labS of Olivier Thibault and Nada Porter .
See: Searcy et al. J Alzheimers Dis. 2012;30(4):943-61. doi: 10.3233/JAD-2012-111661.
Luke Searcy: Thanks! I have skimmed the paper and you have done great job! And I noticed all mice you used were female and wonder whether their estrogen level can affect the result. Could you tell me why you chose female then? Considering electrophysiological study was conducted over their 14 months, is the estrogen effect limited on postmenopausal mice? Or estrogen effect are actually not a big concern because their cycles can be synchronized via group housing?
Thank others also! Previous works are helpful for us!
We used females because that is the greatest number of animals we had at the time. The animals were a gift from another Professor.
Hi Luke, thanks for the ref ;)
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