As we know, hippocampus is a widely recognized area Alzheimer's disease affects, and reportedly makes a difference in conventional cognitive assessments, e.g. Morris water maze. And stereotaxic infusion of abeta oligomer can be used to generate acute AD model of rodent.
But to my surprise, most researches with this acute AD model didn't choose hippocampus as their injected site. Instead they injected abeta i.c.v. and reported this can induce neuronal death in hippocampus[1]. Previous study revealed i.h. may have more sereve impairment in memory[2], so why still people prefer so much i.c.v. over i.h.?
Many thanks for your answer. This will help us decide in our own experiment.
[1] Li L, Xu B, Zhu Y, et al. DHEA prevents Aβ 25–35-impaired survival of newborn neurons in the dentate gyrus through a modulation of PI 3 K-Akt-mTOR signaling[J]. Neuropharmacology, 2010, 59(4): 323-333.
[2] Flood J F, Morley J E, Roberts E. Amnestic effects in mice of four synthetic peptides homologous to amyloid beta protein from patients with Alzheimer disease[J]. Proceedings of the National Academy of Sciences, 1991, 88(8): 3363-3366.
It´s a bright question.
In truth both case are acceptable, when you use i.c.v the ventricle will irrigate all brain parts (expected yes), when you put i.h only the hippocampus will be "treated", of course you need minor concentration of drug in i.h.
I give a suggestion. Compare the cases in your research...Probably your job become so interesting and your results too.
It´s a bright question.
In truth both case are acceptable, when you use i.c.v the ventricle will irrigate all brain parts (expected yes), when you put i.h only the hippocampus will be "treated", of course you need minor concentration of drug in i.h.
I give a suggestion. Compare the cases in your research...Probably your job become so interesting and your results too.
There are several reasons. If you inject in the Hc, you have to inject every time in the same area of the Hc for consistency, which can be difficult, and you cannot inject a lot. Also, you do some damage to the Hc, which can be a problem. An icv injection will be more forgiving, you don't damage the Hc, you can inject more, and it will diffuse to the Hc and other areas. So your choice of injection site will depend of your purpose. If you want to see the effect on the Hc only, you have to inject in the Hc. If you don't mind a more global effect, then icv is your choice.
I think intrahippocampal injections is better because some times you have to inject some drugs such as antagonists so you can inject those drugs i.c.v without any drug interaction in one site .
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