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Questions related from Hao Chen
As we know, hippocampus is a widely recognized area Alzheimer's disease affects, and reportedly makes a difference in conventional cognitive assessments, e.g. Morris water maze. And stereotaxic...
10 October 2015 4,389 4 View
I am recently conducting TUNEL on (perfused) mouse brain cryosection and so frustrated by its edge effect in DNase-treated positive control (The attached photo is a closeup of edge area). Not as...
07 July 2015 3,356 13 View
To distinguish protein splicing isoform seems to be quite difficult by using mass spectrometry, because of the nearly identical sequence of them. And after fragmentation in mass spectrometry,...
06 June 2015 1,442 8 View
I am preparing grip strength test for the first time. According to previous papers, people give three or five trials for each mouse and then these results are averaged to one. Seemingly these...
05 May 2015 1,816 6 View
Two years ago, Zhang et al. found mitophagy or autophagy for mitochondria is protective in the reperfusion phase, while deleterious in the earlier ischemia phase. But at that time, seemingly they...
04 April 2015 9,770 2 View
Gallic acid (GA) and Chlorogenic acid (CGA) are two types of secondary metabolites with good antioxidative activity. Recently I wonder, on mouse neuron culture, how glutamate-induced neuronal...
03 March 2015 9,357 2 View
Five days ago we extracted cortex neurons from mouse embryos and today many cell clusters have arisen. A photo of them is attached below. And we noticed when there was no coverslip in wells, less...
02 February 2015 3,965 13 View
We conducted primary neuron culture from mice embryo cortex recently and met this weird phenomenon: a suspending cloud of sticky white indigestible remnant after typsin incubation. Similar case...
02 February 2015 7,485 11 View
Recently we are desperate about ice-injury of mouse brain slice in HE staining. There are so many small holes in our 8um slice, probably caused by ice-formation. We perfused our mice and used...
02 February 2015 8,660 29 View
It is said that dendritic spines can naturally occur among primary mouse neurons and their density or morphology can be thoroughly assayed. So can anybody provide any information? At the moment we...
01 January 2015 6,983 6 View
According to JAX, it is not recommended to use male 3xTg mice as tested subjects because of the report that male transgenic mice may not exhibit the phenotypic traits originally described. But for...
10 October 2014 5,599 6 View
