How do I assess when to use "match between runs" for LFQ analysis on Maxquant?

Hi Proteomics enthusiasts, I am currently analyzing DNA pulldown data (treated vs non-treated) using Label-free quantification on Maxquant. I am trying to assess if a protein is present in one...

04 August 2020 1,090 6 View

Why am I unable to detect Histone PTMs at their correct molecular weight in nuclear extracts via Western Blotting?

I am trying to detect changes in Histone PTMs with or without treatment in nuclear extracts from cell lysates. I use a Hypotonic buffer (20 mM Tris pH 7.4, 10mM NaCl, 1mM MgCl2, 0.5mM EDTA)...

10 March 2020 2,626 6 View

Conceptual/Theoretical model of participation in mutual fund / microcredit?

I am looking for a theoretical or conceptual model of participation in mutual fund or microcredit group. Although there are several empirical research about drivers and determinants linked to...

11 February 2020 5,179 3 View

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18 June 2019 7,409 8 View

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22 October 2018 3,303 3 View

Does anyone have a Nocodazole release protocol for A549 cells?

I am doing an experiment to investigate the effect of our compound on cell cycle. I will be using Nocodazole to synchronize my A549 cells but see a wide variety of doses when searching the...

01 January 2018 5,973 3 View

How long does it generally take to recieve the full-text article/journal?

I have made several requests this afternoon for full-text articles/Journals. I am a new user to this site and am just curious how long it generally takes to process those requests. Thank you!

11 December 2017 4,893 5 View

How do you reconstruct a 3d image from a single hologram?

Hi there! I have a confusion on the idea of what a 3D image really is from the hologram reconstruction. Sometimes the paper talks about a 3D volumetric image from a stack of amplitude intensity...

22 October 2016 9,709 3 View

Why do most cell lysis buffers for western have either NP40 OR Triton-X? Is there a reason that you can't mix the two into one universal buffer?

11 April 2016 6,568 2 View

Has anyone noticed poor agreement between replicates using the qiagen one-step RT-PCR kit?

I have been using the kit above for some time now, which performs cDNA conversion and normal qPCR in the same tube. I am noticing that despite using recently calibrated pipettes and preparing my...

30 April 2015 5,024 10 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View