We're trying to develop a more humane protocol for perfusion in laboratory mice. We had mice waking up during perfusion from anesthesia with avertin, but not with pentobarbital anesthesia using buprenorphine (for pain control) as a pre-med. Currently there is a shortage/backorder of pentobarbital so we are seeking alternative. We are considering a combination of ketamine and xylazine for anesthesia with buprenorphine + Acepromazine OR Midazolam pre-med.
We like this option because of its welfare benefits: 1) it can be given sub-Q, avoiding painful/uncomfortable IP, and 2) it provides excellent pain control.
But we are also concerned about potential effects of the drug combination (especially ketamine) on our outcome measures - primarily markers of neuronal activity and plasticity (e.g. IEGs, dendritic spine density, BDNF etc. though we are not sure precisely which assays we will be running yet).
Thank you all very much for your help!
I believe these drugs can dramatically interfere with neuronal activity and plasticity.
Best Regards
Carlos
As far as my concern ,ketamine (NMDA antagonist) and Xylazine can provide excellent analgesia but These drugs may causes neuronal changes as glial morphology as well as physiological alterations.
If wish to avoid the KETAMINE /XYLAZINE with impact on NEURONAL tissue, Other Options will be to use Propofol or Benzodiazepines along with Narcotics for inducing Anaesthesia for the Experimental animals.
I do not consider it advisable: There are multiple better options
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