16 Questions 74 Answers 0 Followers
Questions related from Zhi Xiong Chong
Hi all, Recently I encountered some irregular black/grey patches inside my cell culture flask (see attached image, x400 magnification). They are not motile, not floating, do not stick to cells...
09 July 2020 8,204 3 View
Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...
22 December 2019 9,923 5 View
Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...
20 September 2019 3,182 2 View
Hi all, I am currently using Hirschmann hemocytometer (neubauer improved) for cell counting. I am planning to buy another one in case the current one breaks.. Which brand do you recommend?
19 September 2019 9,355 3 View
Hi all, I am facing the problem with the amplification of the non-template control (NTC) towards the end of the qPCR cycle (SYBR Green based qPCR). I ran 40 cycles and the NTC amplification...
28 July 2019 2,698 6 View
Hi, Can I autoclave glycerol for more than 1 time? Thank you.
30 June 2019 3,917 1 View
Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.
18 June 2019 1,316 3 View
Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...
15 June 2019 5,556 6 View
Hi all, I am planning for some transfection work involving a mammalian cancer cell line. Can I know the transfection kit from Qiagen or ThermoFisher lipofectamine is a better option? Do you...
24 March 2019 10,021 3 View
Hi all, I am going to start some cloning and transfection works involving the human breast adenocarcinoma cell line, MDA-MB-231. I have some questions as shown below: 1) Does cryopreservation...
21 March 2019 4,883 0 View
Dear Scientist, I am looking for constructive feedback on the possible causes of pale shady band below the desired band on agarose gel. Kindly refer to the photo attached and you may find pale...
16 December 2018 4,571 2 View
Hi all, Recently I have been trying to sequence a local edible mushroom strain and it was found that the results were not really good due to many background noise and I was told that this might...
21 February 2018 7,033 8 View
Nicotinic acetylcholine receptor is an ion gated channel and I would like to know whether the allosteric modulation of this receptor will affect the GIT motility. As GIT is innervated by...
10 December 2017 8,728 0 View
In a concentration-response curve, both control and drug X almost stay at the same position and have same slopes. Is there a way to determine CR of X? I was told that X could be a competitive...
10 December 2017 8,109 0 View
Hi all, Recently I was recommended to use QIAGEN miRCURY LNA miRNA mimics / inhibitors in transfecting mammalian cancer cell lines. I was told that the mentioned mimics / inhibitors can be...
01 January 1970 6,837 6 View
Hi all, I am working on transfection experiments involving miRNA in mammalian cell lines and I have a doubt here. After optimizing my experiment, I am able to achieve good transfection efficieny...
01 January 1970 1,101 4 View
