Why the velocity of the spray droplets are not matching based on two diagnostics techniques (Particle image velocimetry (PIV) and PDPA?

Why the velocity of the spray droplets are not matching based on two diagnostics techniques (Particle image velocimetry (PIV) and Phase Doppler particle analyzer?

03 October 2019 5,100 9 View

How to analyse tafel curve in primary Zn air batteries?

Would you please suggest me any analysing technique for tafel curve in the primary Zn air battery device. And which instruments do I need to perform to get the tafel curve for my material?. If you...

22 April 2019 8,429 3 View

Which journals (SCI indexed) are suitable for submitting paper on the chracterisation and evaluation of dissimilar joints related research?

details about the journals for the publication of research on dissimilar joints.

04 April 2019 9,244 3 View

Is it possible to coat pure aluminium on Stainless steel?

i would like to coat pure AL. on stainless steel, which method is good for the above said to improve strength.

14 June 2018 2,534 8 View

Can any one suggest me some way to find annual growth line in freshwater bivalves(unionidae)?

11 September 2017 4,408 3 View

CDNA conversion kit (QuantiTect Reverse Transcription Kit-Qiagen) can be used for cloning purpose of Human gene?

My purpose is to clone a human target gene (for eg:insulin gene) into a cell line and express the same in cell line for research purpose. My plan is to take RNA from human tissue and convert it to...

23 February 2017 4,101 1 View

How to apply the proposed architecture for image processing applications?

we have designed a proposed multiplier using Verilog HDL code. Now we want to apply our proposed multiplier for image processing applications. We need procedure or steps or example codes. Can...

20 March 2016 4,086 4 View

CDNA or DNA quantification reads A260/280=2.0 with 10ng/mu L and above will affect the Real Time PCR ?

09 March 2016 1,329 2 View

How to find the release of drug for different PH values?

03 February 2016 2,899 2 View

How can I Implement DCT using proposed multiplier?

Can any one provide me the verilog code to calculate PSNR value for my proposed system. If any one helping the manual (steps) for image processing application, it is highly appreciated

21 December 2015 4,744 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View