Why the velocity of the spray droplets are not matching based on two diagnostics techniques (Particle image velocimetry (PIV) and PDPA?

Why the velocity of the spray droplets are not matching based on two diagnostics techniques (Particle image velocimetry (PIV) and Phase Doppler particle analyzer?

03 October 2019 5,100 9 View

How to analyse tafel curve in primary Zn air batteries?

Would you please suggest me any analysing technique for tafel curve in the primary Zn air battery device. And which instruments do I need to perform to get the tafel curve for my material?. If you...

22 April 2019 8,429 3 View

Which journals (SCI indexed) are suitable for submitting paper on the chracterisation and evaluation of dissimilar joints related research?

details about the journals for the publication of research on dissimilar joints.

04 April 2019 9,244 3 View

Is it possible to coat pure aluminium on Stainless steel?

i would like to coat pure AL. on stainless steel, which method is good for the above said to improve strength.

14 June 2018 2,534 8 View

Can any one suggest me some way to find annual growth line in freshwater bivalves(unionidae)?

11 September 2017 4,408 3 View

CDNA conversion kit (QuantiTect Reverse Transcription Kit-Qiagen) can be used for cloning purpose of Human gene?

My purpose is to clone a human target gene (for eg:insulin gene) into a cell line and express the same in cell line for research purpose. My plan is to take RNA from human tissue and convert it to...

23 February 2017 4,101 1 View

How to apply the proposed architecture for image processing applications?

we have designed a proposed multiplier using Verilog HDL code. Now we want to apply our proposed multiplier for image processing applications. We need procedure or steps or example codes. Can...

20 March 2016 4,086 4 View

CDNA or DNA quantification reads A260/280=2.0 with 10ng/mu L and above will affect the Real Time PCR ?

09 March 2016 1,329 2 View

How to find the release of drug for different PH values?

03 February 2016 2,899 2 View

How can I Implement DCT using proposed multiplier?

Can any one provide me the verilog code to calculate PSNR value for my proposed system. If any one helping the manual (steps) for image processing application, it is highly appreciated

21 December 2015 4,744 2 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

How long can you store Matrigel-coated Transwell inserts?

Hi, everyone! I was planning to do an invasion assay today and thus coated my inserts yesterday, but unfortunately, due to certain circumstances, I cannot proceed. The next time that I can do the...

09 February 2021 8,771 3 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Spontaneous metastasis of nsg mice reason for early metastasis?

Hello. I have injected breast cancer cells with matrigel in 4th inguinal mammary fat pad of nsg mice. i was not expecting the cells to metastasize before 2 weeks but when I imaged them on day...

31 January 2021 2,817 2 View

How to do immunostaining with 3D culture (tissue embedded in matrigel)?

I dissected tissue from mice then embedded it in matrigel for culture. For analysis, I plan to do immunostaining with some cell markers. But the regular IF protocol did not work. Matrigel would...

11 January 2021 6,961 3 View

Des anybody have a straight forward protocol for invasion assay using colon cancer stem cells? Is there necessary to dilute the Matrigel?

I am working on colon cancer stem cells to see the impact of combinational therapy.

23 December 2020 9,830 3 View

Why the oral cancer cell line from metastatic tongue origin is showing less aggressive property?

I am working with oral cancer cell line SCC090 (ATCC). As per ATCC data sheet it is originated from metastatic tongue. It's primary site is not mentioned. Due to the origination from metastatic...

08 December 2020 5,674 3 View

How do you fix organoids for IHC staining and H&E sections??

I work with ovarian cancer cell line organoids embedded in matrigel, they grow well the A2780 cells, but once I start using 10% formaldehyde to fix them they disintegrate...does anybody have a...

29 November 2020 1,900 1 View

Whats the Alternate for cell recovery solution from matrigel ??

Hello, my work involves growing cells in the matrigel and for recovering cells from matrigel i usually use cell recovery solution from corning. As now i am curious to know if theres an alternate...

20 November 2020 1,148 3 View