Considering the long back log for the order of Claycomb Media, I am looking for substitution of Claycomb Medium. Has anyone successfully cultured HL-1 cells using DMEM/ OPTIMEM supplemented with Norepinephrine, Retinoic acid?
In my data set, T is greater than N, so I chose quantile regression for my data set. So is it appropriate for that?
15 July 2024 6,416 4 View
Suppose, a variable like FDI has no data over a period of time in a country. At that time, how can we run our model on panel data?
25 June 2024 7,162 1 View
My friend who is a Credit Review Officer is working on a research project. The outcome need to be published hence facing difficulties. Bank of Bhutan is not allowed in institution details. Any...
06 June 2024 990 2 View
I am creating enzyme variant library through random mutagenesis but I am not getting any colonies after transformation using ligated product. I have E. coli BL21 (DE3) and BL21 Rosetta 2 (DE3)...
27 May 2024 5,332 0 View
Filling research gap takes more years. It is not always easy .
06 May 2024 4,281 1 View
I have installed a linux system for the same purpose and downloaded the VIC-4.2.d model. While looking for any tutorial series and manual for use and operation of VIC is not found. I would be very...
29 April 2024 9,097 1 View
In spinel ferrite-type samples we can see this kind of cases.
31 March 2024 5,259 2 View
ANN- Artificial Neuron Network. I want to implement ML for faster result so seeking guidance
15 March 2024 9,710 1 View
I have been trying to measure the size of the human lysozyme protein with DLS. The instrument is Otsuka ELS-Z 1000. The upper and lower measurement size limits are 10 nm and 4000 nm respectively,...
18 February 2024 9,885 3 View
I want to know proper process for the concentration of gasoline present in soil and after removal of gasoline, how much quantity left in the soil. So, can anyone please tell me the detailed...
05 February 2024 9,718 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View