Has anyone been successful with anti pSTAT3 Ab (Cell signaling #9145) in paraffin IHC mouse lungs?

10 January 2018 0 2K Report

Hello,

I'm having trouble getting a positive signal using the Cell Signaling Ab anti)phosphor STAT3 (#9145) in a paraffin embedded mouse lung section.

Have anyone got a simple and convenient positive control I can use?

Thanks.

Badges
Science topic

More Alexis Broquet's questions See All

Cell culture model of Acute kidney injury?

Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...

29 July 2024 5,622 3 View

Assistance with high taxonomic level identification of intertidal microalgae in SEM Images?

I am currently conducting research on benthic microalgae in the intertidal zone along the central California coast, specifically focusing on grazing by marine snails. As part of this study, I have...

08 July 2024 3,342 5 View

To what extent should "reading comprehension" be analyzed in an A.R. based on phonological awareness instruction to EFL students using a webtool?

Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...

02 July 2024 8,480 0 View

What are the mechanisms involved in which bacteria gains resistance against antibiotics?

An important factor to consider when providing antibiotics is the possibility of the bacteria gaining resistance to it. My questions pertains to how these bacteria are able to gain resistance and...

06 February 2024 5,153 2 View

Do you have an opinion on the HI-98194 Multiparameter Waterproof Meter by Hanna Instruments?

longevity, solidity, patency, reliability.... Thanks

24 January 2024 8,325 0 View

To what extent does the use of Quizlet during the pre-reading stage support 4th graders’ ability when reading for gist?

Hello, I am attempting to conduct action research based on the following research question. Could you please provide any advice, authors or research field to deeper exploring? Thanks in advance....

25 December 2023 7,097 8 View

Dear all, how do I access the QA metadata for MOD13A2 composites?

I'm trying to obtain the the quality assurance metadata for MOD13A2 MODIS 16-day composites. Any help will be greatly appreciated.

24 October 2023 353 0 View

Was the king in Ancient Egypt subject to judgment after death?

James Allen (and others) just assert this, but Brandon in the 1950's was not sure, and it does not seem so clear to me either. The Instruction of Merykare seems rather clear that there is a...

03 October 2023 8,463 2 View

How can I prevent cannula exposure of Alzet osmotic pumps?

I am working to establish a protocol for ICV drug administration via an Alzet osmotic pump brain infusion kit using mice. Although, this is a well-established technique, I continue to have issues...

06 July 2023 8,300 0 View

Calcular la velocidad específica de crecimiento de L.casei, en los dos reactores. b) Calcular la X que se produce en cada reactor?

Se tiene el microorganismo Lactobacillus casei que se caracteriza por una cinética del tipo Monod con los siguientes parámetros: μmax = 0.2 hr -1 Y x/s = 0.5 kg biomasa/kg sustrato Ks = 1,5 kg/ m...

28 March 2023 7,376 0 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Can I separate xylene from paraffin after deparaffinization of microtome samples and reuse the xylene?

...

24 July 2024 1,559 1 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

Do we put respondents data in research methods or findings section of a qualitative study?

Are both ways acceptable, or is there any convention to them? Thanks.

22 July 2024 6,988 4 View

Radar Detection Probabilities using beta distributed Scattering Cross section?

Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...

22 July 2024 868 0 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View