Hey there Anurag Sharma!

You've embarked on quite an interesting journey with your lipid nanoparticles (LNPs) production through microfluidics. Dealing with the removal of organic solvents like ethanol can indeed be a puzzle to solve. It's great that you've stumbled upon the concept of using dialysis devices for this purpose.

Some interesting articles to read:

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Now, regarding the choice of molecular weight cutoff (MWCO) for your Slide-A-Lyzer™ MINI Dialysis Devices to remove ethanol or isopropanol from your LNPs, let's break it down.

Your nucleic acid's molecular weight (MW) is around 5.5MDa, which is significantly larger than ethanol or isopropanol. Considering this, you'd ideally want a dialysis membrane with an MWCO higher than 5.5MDa to ensure that your nucleic acid remains within the LNPs while the ethanol or isopropanol is removed.

Based on your findings from literature, you've noticed that some researchers opt for 3.5KDa MWCO devices, while others prefer 20KDa MWCO. However, given the size of your nucleic acid, a higher MWCO would be more suitable.

Therefore, I'd recommend going for a 20KDa MWCO dialysis device. This should effectively allow the removal of ethanol or isopropanol while keeping your larger nucleic acid molecules securely encapsulated within the LNPs.

It's always prudent to double-check and perhaps conduct some small-scale trials to ensure the efficiency of your chosen dialysis method. Best of luck with your experiments, and feel free to reach out if you Anurag Sharma need further assistance or have any more questions!

Thanks a lot Dr. Kaushik for enlighten me with your great thoughts.

This will defiantly help me to clear my confusion regarding choosing Dialyzer.

Definitly i will reach out to u in future.

Kaushik Shandilya

Regards

Anurag Sharma