Is there any databases of sequencing data for AD mouse models?

I am working with the NL-G-F and 5x FAD mice. Are there any sequencing databases publicly available to mine for brain or retina? Thanks!

20 January 2021 6,425 1 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

What are the applications of remote sensing and GIS in transportation, what are the types of sensors you will require?

How do you use Remote sensing and GIS in transportation, what are the types of images you will require, what will be their wavelength, what type of sensors will you require?

20 December 2020 6,375 5 View

Non-biological recognition elements for bacterial sensors?

Hi, I am interested in non-biological recognition elements for bacterial sensors. I am aware of molecularly imprinted polymers but I do not seem to find many other alternatives. I know that many...

18 September 2020 5,894 3 View

Linear Mixed model analysis - assessing baseline covariate?

I am analysing treatment group differences at time 2 (two time points only) data on a psych measure outcome (with five covariates: age, gender, CVD, BMI and F2s), and I am interested in one of the...

25 August 2020 6,904 3 View

Autoclaving Powder Skimmed Milk?

Hello! Does anyone has a effective way to autoclave 10% skimmed milk without caramelization? I tried 121C for 15min (in the powder form) but I am struggling with the color which is undesirable....

30 June 2020 4,601 3 View

Technique to estimate the amount of nanoparticles in a coating?

Hi everyone, I am coating stainless steel with 10%PLA and Nanoparticles, and I am trying to find out techniques that I can use to characterize the coating surface aside from SEM and to quantify...

08 June 2020 2,293 7 View

How to inactivate SARS-Cov-2 in tissue for spatial transcriptomics?

We want to run 10x Genomic Visium spatial transcriptomics on lung tissue from COVID-19+ patients. How can we inactivate the virus so it's safe to work with the tissue at BSL2? The first step is...

28 April 2020 4,704 3 View

How can one perform spin coating of stainless steel 316L with a polylactide-chloroform solution?

I am trying to do for my thesis a uniform homogeneous coating with PLA (polylactic acid with 1.05 dl/ g viscosity) in Stainless steel 316L medical grade via spin coating, but I haven't been able...

07 April 2020 8,556 3 View

What is the mutation rate in the mtDNA of the Lepidoptera?

I want to determine the phylogenetic age of the members of the Lepidoptera (Sphingidae) based on mtDNA, using BEAST software; But I do not have a good indicator. Please what is mutation rate in...

28 March 2020 1,559 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View