One of the tests performed to check the polyspecificity of an antibody selected in vivo or in vitro is to test it in ELISA against BVP extract. My idea is to start already during the selection campaign to add the BVP extract as a blocking reagent for my phage libraries.
Hallo Fortunato,
you can actually, use one blocking well with your BVP coated extract on which you can pre-incubate your phage libraries. I did a similar experiment in which I didn't want cross-reactive antibodies against a specific cell protein. So, I used the cells as blocking material for the pre-incubate step and afterwards transferred my libraries to my target antigen. Nevertheless, I would suggest to test the selected antibodies for cross-reactivity in a screening ELISA.
Best wishes
Stephan
I usually use biotinylated-antigens in solution and magnetic beads for the actual selection, so I was wondering if I can add the BVP directly in the phage solution, but it is not too much work to preclear the phage before passing them through an ELISA plate well or an immunotube coated with BVP
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