13 March 2014 2 8K Report

To detect human IgG and IgA from serum samples in flow cytometry.

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Why are there markers in my Emotiv recordings that I did not send?

I am currently using an emotiv epoc x headset for research into SSVEP signals, and I have my stimulus program sending a marker value of 1 at the beginning of the stimulus, and a marker value of 2...

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Can dry ice shipment affect the integrity of skin tissues fixed in 10% NBF and stored in 70% ethanol?

Hi to everyone. Recently I received skin tissues stored in 70% ethanol upon fixation in 10% NBF that would need to be processed for paraffin embedding. The samples have been sent in dry ice,...

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Do you know a reference on action of Heisenberg-Weyl algebra on some basis states?

I am looking for references on Heisenberg-Weyl algebras, namely I am interested in the action of algebra elements on some basis states, i.e. matrix elements in a certain basis.

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Has anyone used recombinant proteins provided by mybiosource.com?

There is a recombinant protein of interest that they provide which I might be interested to use in phage selections.

12 February 2020 3,556 2 View

Can I sum confidence intervals of two population attributable fractions (PAFs)?

If I want to know the impact of two mutually exclusive risk factors on disease burden in my population using evidence-based PAFs and their 95% CIs, can I sum the total numbers of cases (estimates)...

03 February 2020 5,664 5 View

How can I transfer a small molecule from a surface into a cell?

I am dealing with support-mediated drug delivery systems, and in particular I have some cancer cells adherent on a collagen surface. I loaded a hydrophilic drug on collagen by physisorption, then...

03 August 2019 326 4 View

What is the best FACS out there?

I have been using FACAria II (upgraded later to III) for the last 9 years to sort from yeast, to bacteria to mammalian cells. Specially after the fluidic upgrade, I really cannot complain about...

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Which is the best mRNA purification kit?

I would like to have an updated answer to this question. I am looking for a easy but high yield kit (Miltenyi? Qiagen? Dynabeads?) Has anyone experience with the Miltenyi one? Thanks

31 October 2016 5,457 5 View

Why my recombinant polyclonal antibodies don't recognize their target in WB?

We produce in vitro recombinant antibodies for different targets. When tested in ELISA -using folded target proteins - they all specifically recognize their target, but around 25-30% of them don't...

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