Desalting using TCEP?

Hi all, Currently working on in-gel digestion for peptide identification, other than using ziptip or centrifugal unit to desalt sample, I heard using TCEP also works well to desalt. I wish to...

03 April 2019 8,110 4 View

Cell culture techniques?

Hi all, Just out of curiosity, is there any ways to culture cell line which goes from suspension to adherent type? I am actually culturing fish spleen cells however, it does not appear attached...

10 November 2017 9,710 4 View

Membrane tracker

Hi all, I am currently expressing a membrane protein on eukaryotic cell line, I tried to use CM-DiI for membrane labeling to verify the localization of my target protein, however, effect of CM-DiI...

06 July 2017 929 1 View

Any references on what benefits mycorrhizal fungi(AMF) obtained when colonized with plants?

10 November 2016 4,937 0 View

Low competent cell growth rate?

Hi all, i am currently using rosetta competent cell to expressing my target protein, I incubated to OD~0.4, using IPTG induction in 25C overnight, however the next day OD only ~0.5, it did not...

10 November 2016 4,869 2 View

Tracker for membrane protein?

09 October 2016 4,049 4 View

Receptor protein purification difficulties?

08 September 2016 8,247 13 View

Protein expression of a type of receptor?

Hi all, I am currently trying to express a type of cell-surface receptor(full length) using E.coli bacteria, however, i only can get small amount of protein expressed after ran SDS-PAGE gel, i...

07 August 2016 2,436 7 View

Haemagglutinin or neuraminidase?

What is differences between expression of haemagglutinin or neuraminidase surface antigenic in influenza viruses? Expressing recombinant HA have better antigenicity than neuraminidase?

04 May 2016 6,949 2 View

Plasmid construction for receptor type?

04 May 2016 3,069 1 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View