I am learning to construct plasmid using pET29 but there are 3 different types, what is the differences between pET29a/pET29b/pET29c?
The difference is at specific sites which have been changed. Very similar to pET28 system. You can clarify with the following link.
http://www.synthesisgene.com/vector/pET-29a.pdf
Dear Tang,
The a,b,c annotations denote consecutive reading frames relative to the BamH I cloning site recognition sequence, GGATCC.
Thank you very much for the clarification.
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