Desalting using TCEP?

Hi all, Currently working on in-gel digestion for peptide identification, other than using ziptip or centrifugal unit to desalt sample, I heard using TCEP also works well to desalt. I wish to...

03 April 2019 8,110 4 View

Cell culture techniques?

Hi all, Just out of curiosity, is there any ways to culture cell line which goes from suspension to adherent type? I am actually culturing fish spleen cells however, it does not appear attached...

10 November 2017 9,710 4 View

Membrane tracker

Hi all, I am currently expressing a membrane protein on eukaryotic cell line, I tried to use CM-DiI for membrane labeling to verify the localization of my target protein, however, effect of CM-DiI...

06 July 2017 929 1 View

Low competent cell growth rate?

Hi all, i am currently using rosetta competent cell to expressing my target protein, I incubated to OD~0.4, using IPTG induction in 25C overnight, however the next day OD only ~0.5, it did not...

10 November 2016 4,869 2 View

Tracker for membrane protein?

09 October 2016 4,049 4 View

Receptor protein purification difficulties?

08 September 2016 8,247 13 View

Protein expression of a type of receptor?

Hi all, I am currently trying to express a type of cell-surface receptor(full length) using E.coli bacteria, however, i only can get small amount of protein expressed after ran SDS-PAGE gel, i...

07 August 2016 2,436 7 View

What is the differences between pET29a or b or c? Which would give best result for plasmid construction of receptor type?

I am learning to construct plasmid using pET29 but there are 3 different types, what is the differences between pET29a/pET29b/pET29c?

06 July 2016 2,211 3 View

Haemagglutinin or neuraminidase?

What is differences between expression of haemagglutinin or neuraminidase surface antigenic in influenza viruses? Expressing recombinant HA have better antigenicity than neuraminidase?

04 May 2016 6,949 2 View

Plasmid construction for receptor type?

04 May 2016 3,069 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

No title

01 March 2021 3,905 5 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View