12 Questions 9 Answers 0 Followers
Questions related from Tang Zhi Zhuang
Hi all, Currently working on in-gel digestion for peptide identification, other than using ziptip or centrifugal unit to desalt sample, I heard using TCEP also works well to desalt. I wish to...
04 April 2019 8,207 4 View
Hi all, Just out of curiosity, is there any ways to culture cell line which goes from suspension to adherent type? I am actually culturing fish spleen cells however, it does not appear attached...
11 November 2017 9,812 4 View
Hi all, I am currently expressing a membrane protein on eukaryotic cell line, I tried to use CM-DiI for membrane labeling to verify the localization of my target protein, however, effect of CM-DiI...
07 July 2017 1,008 1 View
Hi all, I am currently reading journals regarding arbuscular mycorrhizal fungi, in this symbiosis relationship, what kind of benefits AMF obtained from plants? As most of papers discussed only...
11 November 2016 5,015 0 View
Hi all, i am currently using rosetta competent cell to expressing my target protein, I incubated to OD~0.4, using IPTG induction in 25C overnight, however the next day OD only ~0.5, it did not...
11 November 2016 4,939 2 View
Hi all, I am currently expressing a membrane protein on eukaryotic cell line, the morphology observed have similarities with other eukaryotic cell line expressing same protein, but is there any...
10 October 2016 4,123 4 View
Hi all, I successfully expressed receptor protein in e.coli expression system, and its in soluble form however, when i proceed to purify it, there is no binding to the column(current using...
09 September 2016 8,329 13 View
Hi all, I am currently trying to express a type of cell-surface receptor(full length) using E.coli bacteria, however, i only can get small amount of protein expressed after ran SDS-PAGE gel, i...
08 August 2016 2,521 7 View
I am learning to construct plasmid using pET29 but there are 3 different types, what is the differences between pET29a/pET29b/pET29c?
07 July 2016 2,339 3 View
Hi, i wanna ask if there any vectors can be use to ligate 1.4 kb fragment other than pgem T-easy or T vector?
05 May 2016 3,535 6 View
Hi all, What would be the best choice if i want to construct plasmid with only extracellular domain of a receptor? Is there any rules as i want to avoid transmembrane region..
05 May 2016 3,138 1 View
What is differences between expression of haemagglutinin or neuraminidase surface antigenic in influenza viruses? Expressing recombinant HA have better antigenicity than neuraminidase?
05 May 2016 7,114 2 View
