Is it possible to use a HA peptide signal for a membrane protein expression in HEK ? can it have a deleterious effect for the protein (toxicity)?
I know it is used for Sf9 expression but I can't find any information about mammalian cells.
Krzysztof Mikolajczyk
Dear C. Lea,
yes, you can use HA-tag, I do not know about any toxicity effects of this epitope in HEK cells.
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Conchou Léa
Krzysztof Mikolajczyk thank you for your answer, in this case I was interested of using a HA-signal peptide instead of a HA-tag, to help address the protein to the membrane .. usually it used with Sf9 expression
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