I have been working on strain selection and improvement in Ulva spp. Since we have several strains differing in their growth patterns under same (controlled) culture conditions, I am interested in studying growth kinetics of fast as well as slow growing strains.
Although, I have some knowledge about setting of these experiments, I need advice from the experts - even those working on other organisms - who are involved in studying kinetics.
Anticipating references and suggestions.
I have worked with Ulva spp in the field quite a bit with the lactua type morphology. These are broad yet thin (2 cells wide) but can triple their tissue N in a few days when placed in coastal water with anthropogenic N sources.
We grow Ulva in the laboratory, regularly in batch cultures and in a simple flow through system. We use Provasoli Enriched Seawater media which has high nutrient content and light intensities above 500 micro moles of photons /m2/s to get the highest growth rates. More water motion is better as long as the thalli aren't breaking.As you might already know, the thalli can sporulate with little warning if the conditions change abruptly and also on a semilunar cycle.
The easiest metric of growth is change in biomass. However, you may find that it does not capture all of the variation between your strains if the surface area to biomass ratio or the nutrient content of the biomass differs.
Hi Janet:
Thanks for sharing your experience. I am more specifically looking at 'kinetics of growth' under any laboratory conditions. I also would like to know, how growth matrix changes over the period of time. If there are any publications or even un-published results in the form of report, you may share.
Vaibhav
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