I am going to be culturing BV-2 with a CHO (Chinese hamster ovarian) line.
Would be grateful if anyone could provide information on inserts and wells that have been good with either of these cells and which pore size is best for protein movement. As I want the protein they are genetically engineered to express to pass through.
The best ones I have found are Corning. But the link for them below but what is the best pore size if I want amyloid beta to get through. As from reading papers they said 0.4 - 3um but don't know which is better and as they are quite expensive only want to get the right ones.
https://www.fishersci.co.uk/shop/products/family/176705?gclid=CjwKCAiAjPyfBhBMEiwAB2CCIpKUtoe8ik49MRhaobwGFxTLDE6WomSL7DqBydBs9WsRTC_QG3stJBoCaJcQAvD_BwE&ef_id=CjwKCAiAjPyfBhBMEiwAB2CCIpKUtoe8ik49MRhaobwGFxTLDE6WomSL7DqBydBs9WsRTC_QG3stJBoCaJcQAvD_BwE:G:s&s_kwcid=AL!4428!3!396451659981!!!g!!&ppc_id=NonBrand_goog_8076196020_88792043172_DSA__396451659981_14381467006670270117&s_kwcid=AL!4428!3!396451659981!!!g!!#tab
Thanks for any help you can give.
Best wishes,
Abdullah