23 September 2023 3 7K Report

I am trying to express several proteins at the same time, but I want to use a different promoter and terminator for each one to avoid the possibility of recombination.

The promoters that are available to me are: TEF2, PGK1, CCW2, TDH3 and HHF2. The available terminators are: ENO1, SSA1, ADH1, PGK1 and ENO2.

Has anyone ever used these combinations of promoters and terminators? In your experience, which combinations work the best?

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