Vanja Jurić

6 Questions 2 Answers 0 Followers

Questions related from Vanja Jurić

I need a buffer for yeast cells that can maintain pH 9. What would you recommend?

I tried glycine buffer but the yeast cells acidify the buffer so the pH goes down to 7-6 overnight. I need something that will stay around 9 and that isn't toxic to the cells.

19 April 2024 7,974 4 View

Good promoter terminator combinations for protein expression in Saccharomyces cerevisiae?

I am trying to express several proteins at the same time, but I want to use a different promoter and terminator for each one to avoid the possibility of recombination. The promoters that are...

23 September 2023 6,791 3 View

Are there any user-friendly tools to predict disulfide bonds from a protein sequence?

I have a protein sequence with two cysteine residues and I would like to predict if those cysteins will form disulfide bonds. I am looking for user-friendly tools to do this, either online tools...

19 January 2023 1,496 4 View

Tips on expressing, extracting and purifying nanobodies in E. coli?

I am trying to express and purify nanobodies from a nanobody library in E. coli. I am using a pBAD vector to which I added the pelB sequence for periplasmic localization. The nanobody is fused to...

02 November 2022 9,859 3 View

How to subtract background signal or outlier events in FlowJo plots?

I used unlabeled cells (first image) to set the gate and the second image is my positive control. I am looking for percentage of green cells (FL-1, ''expressing'' cells in my experiment) that are...

11 October 2022 9,022 2 View

Do you know of any examples of protein-protein interactions that can be disrupted by a small molecule ligand?

One example would be MBP and MBP-74, an interaction which can be disrupted by maltose when it binds MBP.

01 January 1970 1,548 4 View