to use warthons gel for culture we need to sterilize, what form of sterilization is most suitable as we aim to avoid contamination and on the other side avoid denaturation.
perhaps B-propiolactone
Quels sont les mécanismes et les tests syntaxiques, lexicaux et sémantiques du figement des locutions adverbiales?
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Multipotent stem cells have many origins like bone marrow ,adipose tissue, dental pulp etc., but in particular patient which is more beneficial? how can we assess?
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I discovered a family from Iraq with congenital methemoglobinemia that appears to be autosomal dominant trait presenting with polycythemia , cyanosis and neurological manifestations ,i found an...
23 November 2022 9,382 0 View
One of the main reactions considered to produce silicon nitride compound is the chemical reaction of silane and ammonia gases as follows 3SiH4 + 4NH3 = Si3N4 + 24H2 When these precursors are used...
23 July 2021 8,879 3 View
What the the standard and recommendation for data anonymization with consideration to dataset traceability
23 March 2021 549 2 View
Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...
25 January 2021 7,937 3 View
some times vaccinated the flock but the infection occur
15 November 2017 1,311 41 View
there are many ways of burying human body after death according to religion and some burn the body out ,,so what is best scientifically?
29 June 2017 4,653 5 View
I would know which one of Emeria strains are used as a vaccinal strain?
15 May 2017 2,799 55 View
If the poultry house 10 m in width and 100 m in length, whats the formalin : potassium permenganat ?
11 May 2017 3,771 31 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View