Good afternoon, have any of you taken a saliva sample directly to amplify DNA?

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Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

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Why Is My mtDNA Ct Value Higher Than Nuclear DNA in qPCR?

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Is it possible that the Earth's axis of rotation could change?

Due to the shifting ocean currents, the planet could be thrown off balance, altering the angle of rotation, and even causing the axis to shift, plunging us into chaos. In reality, it doesn't take...

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Which commercial technology is available ? 4 brain ?

Following https://catniplab.github.io/Tafazoli-LearningControlBrain/ and https://insights.princeton.edu/2020/05/learning-to-control-the-brain/ which commercial technology is available ? Probably...

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How to Freeze Embryos for Spatial Transcriptomics?

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Are there any information about protocols of CRISPR in leishmania strains?

I am looking for a CRISPR protocol for application in leishmania strains

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What could be causing these smears in my PCR electrophoresis gel?

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30 June 2024 4,107 4 View

¿En qué medida las tecnologías educativas están siendo efectivas para promover el desarrollo de habilidades para la vida?

las

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What are those white spots at the end?

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Can Metabolic Disorders cure by medicine,sustitutes,gens,and stemcell excercises etc?

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22 April 2024 1,959 3 View

The conclusion cannot be based on the criminal ruling ?

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Can yo help me solving this problem please??

Oral 2mg diazepam, half-life 20 h, bioavailability 90%, what is the concentration in saliva after 14 h? knowing that the saliva drug concentration is 0.1 of the plasma concentration.

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Hello, does anyone have idea about collection of tears and saliva from mice?

Any procedure or video would be helpful.

26 November 2023 7,459 1 View

What is the purpose of adding salt to extract drugs from saliva matrix?

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19 November 2023 7,437 0 View

Does anyone have a very good and reproducible protocol for isolating total microbial RNA from saliva?

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23 July 2023 3,957 0 View

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Can saliva be used as an alternative to human serum or plasma in the toxic drug monitoring analytical method for antiepileptic drugs?

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How can I convert Cq in RT-PCR to number of starting copies?

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Yi Meng

how is the result of pre-experiment?

Alexander Sasha Vlassov

viral RNA purification gives best results, but direct SARS-CoV-2 detection in saliva and swabs/transport media- is also possible. The sensitivity is lower though, and there is quite a bit of sample-to-sample variation (due to presence of misc inhibitors etc). There are several options to "lyse" the sample- to release viral RNA for qPCR analysis- heating, chemical or enzymatic treatment