What is the porosity of 1% agarose gel (low melting)?

01 February 2017 8,395 2 View

Looking for surface E.coli antibody?

hello, Iam not expert in bacterilogy, iam looking for specific surface antibody against this particular E.coli strain (MG 1655),it expresses GFP? .my aim is to bind/adhere the whole bacteria on...

07 August 2016 183 20 View

How do I permeabilize cells?

Is there any protocol or method to permeabilize suspension cell (k562 cell) without fixing them? I want to do live cell imaging hence I don't want any fixation?

11 December 2014 6,794 5 View

Whcih Biotin-conjugated antibody can I use for k562 cell surface proteins?

I would like to have biotin on the k562 cell surface for binding studies, and I want specific binding of biotin, hence could anyone suggest which kind of antibody (conjugated with biotin) I can use?

04 May 2014 6,050 5 View

Can we biotinylate surface of E.coli cell?

If yes, can you suggest a protocol?

06 July 2013 6,386 0 View

How to synthesize gold nanorods of 100nm long and 20nm wide?

How can i synthesize negatively charged gold nano rods of 100nm long and 20nm wide?

09 October 2012 791 0 View

How does one make gold coated microstructures hydrophillic?

I have a gold coated microstruture (through spattering). After I remove them from substrate (glass) and transfer them onto another glass slide, how can I make them hydrophillic to bind them to the...

05 June 2012 2,877 6 View

Gold nanoparticle aggregation of glass substrate

Hello i would like aggregate my gold nanoparticles (70-80 nm) on glass slides, usually when the gold particles are on the glass we see a nice plasmon peak depending upon the size of the particles...

02 March 2012 1,213 13 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View