If yes, can you suggest a protocol?
01 February 2017 8,395 2 View
hello, Iam not expert in bacterilogy, iam looking for specific surface antibody against this particular E.coli strain (MG 1655),it expresses GFP? .my aim is to bind/adhere the whole bacteria on...
07 August 2016 183 20 View
Is there any protocol or method to permeabilize suspension cell (k562 cell) without fixing them? I want to do live cell imaging hence I don't want any fixation?
11 December 2014 6,794 5 View
I would like to have biotin on the k562 cell surface for binding studies, and I want specific binding of biotin, hence could anyone suggest which kind of antibody (conjugated with biotin) I can use?
04 May 2014 6,050 5 View
Can someone give a protocol for how to make gold nano stars ?
04 May 2013 5,995 8 View
How can i synthesize negatively charged gold nano rods of 100nm long and 20nm wide?
09 October 2012 791 0 View
I have a gold coated microstruture (through spattering). After I remove them from substrate (glass) and transfer them onto another glass slide, how can I make them hydrophillic to bind them to the...
05 June 2012 2,877 6 View
Hello i would like aggregate my gold nanoparticles (70-80 nm) on glass slides, usually when the gold particles are on the glass we see a nice plasmon peak depending upon the size of the particles...
02 March 2012 1,213 13 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
