If yes, can you suggest a protocol?
as i calculated i found that the porosity of 1% agorose gel is ~99%? i wonder how much does this 1% makes difference with respect to diffusion of solute inside microfluidic chip
01 February 2017 8,464 2 View
hello, Iam not expert in bacterilogy, iam looking for specific surface antibody against this particular E.coli strain (MG 1655),it expresses GFP? .my aim is to bind/adhere the whole bacteria on...
07 August 2016 264 20 View
Is there any protocol or method to permeabilize suspension cell (k562 cell) without fixing them? I want to do live cell imaging hence I don't want any fixation?
11 December 2014 6,909 5 View
I would like to have biotin on the k562 cell surface for binding studies, and I want specific binding of biotin, hence could anyone suggest which kind of antibody (conjugated with biotin) I can use?
04 May 2014 6,126 5 View
Can someone give a protocol for how to make gold nano stars ?
04 May 2013 6,076 8 View
How can i synthesize negatively charged gold nano rods of 100nm long and 20nm wide?
09 October 2012 899 0 View
I have a gold coated microstruture (through spattering). After I remove them from substrate (glass) and transfer them onto another glass slide, how can I make them hydrophillic to bind them to the...
05 June 2012 2,981 6 View
Hello i would like aggregate my gold nanoparticles (70-80 nm) on glass slides, usually when the gold particles are on the glass we see a nice plasmon peak depending upon the size of the particles...
02 March 2012 1,294 13 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
