Looking for surface E.coli antibody?

hello, Iam not expert in bacterilogy, iam looking for specific surface antibody against this particular E.coli strain (MG 1655),it expresses GFP? .my aim is to bind/adhere the whole bacteria on...

07 August 2016 183 20 View

How do I permeabilize cells?

Is there any protocol or method to permeabilize suspension cell (k562 cell) without fixing them? I want to do live cell imaging hence I don't want any fixation?

11 December 2014 6,794 5 View

Whcih Biotin-conjugated antibody can I use for k562 cell surface proteins?

I would like to have biotin on the k562 cell surface for binding studies, and I want specific binding of biotin, hence could anyone suggest which kind of antibody (conjugated with biotin) I can use?

04 May 2014 6,050 5 View

Can we biotinylate surface of E.coli cell?

If yes, can you suggest a protocol?

06 July 2013 6,386 0 View

Gold nano stars ?

Can someone give a protocol for how to make gold nano stars ?

04 May 2013 5,995 8 View

How to synthesize gold nanorods of 100nm long and 20nm wide?

How can i synthesize negatively charged gold nano rods of 100nm long and 20nm wide?

09 October 2012 791 0 View

How does one make gold coated microstructures hydrophillic?

I have a gold coated microstruture (through spattering). After I remove them from substrate (glass) and transfer them onto another glass slide, how can I make them hydrophillic to bind them to the...

05 June 2012 2,877 6 View

Gold nanoparticle aggregation of glass substrate

Hello i would like aggregate my gold nanoparticles (70-80 nm) on glass slides, usually when the gold particles are on the glass we see a nice plasmon peak depending upon the size of the particles...

02 March 2012 1,213 13 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View