What is the porosity of 1% agarose gel (low melting)?

01 February 2017 8,395 2 View

Looking for surface E.coli antibody?

hello, Iam not expert in bacterilogy, iam looking for specific surface antibody against this particular E.coli strain (MG 1655),it expresses GFP? .my aim is to bind/adhere the whole bacteria on...

07 August 2016 183 20 View

How do I permeabilize cells?

Is there any protocol or method to permeabilize suspension cell (k562 cell) without fixing them? I want to do live cell imaging hence I don't want any fixation?

11 December 2014 6,794 5 View

Whcih Biotin-conjugated antibody can I use for k562 cell surface proteins?

I would like to have biotin on the k562 cell surface for binding studies, and I want specific binding of biotin, hence could anyone suggest which kind of antibody (conjugated with biotin) I can use?

04 May 2014 6,050 5 View

Can we biotinylate surface of E.coli cell?

If yes, can you suggest a protocol?

06 July 2013 6,386 0 View

Gold nano stars ?

Can someone give a protocol for how to make gold nano stars ?

04 May 2013 5,995 8 View

How does one make gold coated microstructures hydrophillic?

I have a gold coated microstruture (through spattering). After I remove them from substrate (glass) and transfer them onto another glass slide, how can I make them hydrophillic to bind them to the...

05 June 2012 2,877 6 View

Gold nanoparticle aggregation of glass substrate

Hello i would like aggregate my gold nanoparticles (70-80 nm) on glass slides, usually when the gold particles are on the glass we see a nice plasmon peak depending upon the size of the particles...

02 March 2012 1,213 13 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View